1KN3
Murine PEBP-2 (phosphatidylethanolamine-binding protein-2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-02-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.488 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.816, 51.401, 79.765 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.21 * |
Rwork | 0.210 |
R-free | 0.26700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bd9 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.490 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.880 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.084 | 0.116 |
Number of reflections | 15638 | |
<I/σ(I)> | 8.5 | |
Completeness [%] | 96.4 | 92.4 |
Redundancy | 4.7 | 3.3 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6 * | 291 | 30% PEG 4000, 200mM sodium acetate trihydrate, 100mM Tris.HCl, pH 8.5, VAPOUR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 7.5 (mg/ml) | |
2 | 1 | 1 | Bis-Tris | 20 (mM) | |
3 | 1 | 1 | 100 (mM) | pH6. | |
4 | 1 | 2 | PEG4000 | 30 (%) | |
5 | 1 | 2 | sodium acetate trihydrate | 200 (mM) | |
6 | 1 | 2 | Tris-HCl | 100 (mM) | pH8.5 |