1KN1
Crystal structure of allophycocyanin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS X200B |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 2 |
Unit cell lengths | 105.300, 105.300, 189.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.200 |
Rwork | 0.193 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB code 1ALL |
RMSD bond length | 0.015 |
RMSD bond angle | 2.900 |
Data reduction software | X-GEN |
Data scaling software | X-GEN |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.082 | |
Number of reflections | 20096 | |
<I/σ(I)> | 15.3 | 2.1 |
Completeness [%] | 87.0 | 65 |
Redundancy | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | Liu, J.Y., (1998) Acta Crystallogr., D54, 662. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | drop | 0.25 (M) | ||
3 | 1 | drop | ammonium sulfate | 0.4 (M) | |
4 | 1 | drop | HEPES | 50 (mM) | pH7.3 |
5 | 1 | reservoir | 0.4 (M) | ||
6 | 1 | reservoir | ammonium sulfate | 1.0 (M) |