1KMT
Crystal structure of RhoGDI Glu(154,155)Ala mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.099, 35.921, 67.506 |
| Unit cell angles | 79.19, 82.66, 76.42 |
Refinement procedure
| Resolution | 25.000 - 1.300 |
| R-factor | 0.15962 |
| Rwork | 0.158 |
| R-free | 0.19500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 2.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.021 * | 0.143 * |
| Total number of observations | 147519 * | |
| Number of reflections | 75214 | |
| <I/σ(I)> | 18.6 | |
| Completeness [%] | 89.0 | 55 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 294 | PEG 4000, Tris-HCl, Lithium sulfate, methylpentane diol, pH 8.5, VAPOR DIFFUSION, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 24 (%) | |
| 2 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | MPD | 2.5 (%) |
