1KMO
Crystal structure of the Outer Membrane Transporter FecA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-09-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97800 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 117.083, 88.086, 94.578 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.710 - 2.000 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 27.200 * |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SnB |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 * | 2.130 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.090 | |
Number of reflections | 62452 * | |
<I/σ(I)> | 11.1 | |
Completeness [%] | 92.3 | 0.71 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 284 | Smith, B.S., (1998) Acta Cryst., D54, 697., Doublie, S., (1997) Methods Enzymol., 276, 532. * |