1KMI
CRYSTAL STRUCTURE OF AN E.COLI CHEMOTAXIS PROTEIN, CHEZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2000-08-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0087 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 163.280, 163.280, 54.133 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| Rwork | 0.279 |
| R-free | 0.29800 |
| Structure solution method | MAD |
| Starting model (for MR) | 1FQW AS A PARTIAL MODEL |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.540 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.074 | 0.457 |
| Number of reflections | 14532 | |
| <I/σ(I)> | 22.3 | 3.1 |
| Completeness [%] | 88.0 | 89.6 * |
| Redundancy | 7.3 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 4 * | isopropanol, ammonium acetate, bicine, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 4 * | isopropanol, ammonium acetate, bicine, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 4 * | isopropanol, ammonium acetate, bicine, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | CheY | 0.264 (mM) | |
| 2 | 1 | drop | 3.6 (mM) | ||
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | CheZ E134K | 0.264 (mM) | |
| 6 | 1 | reservoir | Bicine | 0.1 (M) | pH8.5 |
| 7 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 8 | 1 | reservoir | isopropanol | 30 (%(v/v)) |
