1KMH
Crystal Structure of spinach chloroplast F1-ATPase complexed with tentoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-06-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8452 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 146.887, 146.887, 381.679 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 3.400 |
| R-factor | 0.29788 |
| Rwork | 0.297 |
| R-free | 0.31895 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fx0 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.995 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.089 | 0.383 |
| Number of reflections | 19747 * | |
| <I/σ(I)> | 9.43 | 2.51 |
| Completeness [%] | 92.5 | 79.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.5 | 293 | Lithium sulfate, HEPES, dithiotreitol, pH 7.5, MICRO BATCH, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 0.05 (mM) | |
| 2 | 1 | 1 | HEPES | 25 (mM) | pH7.5 |
| 3 | 1 | 1 | dithiothreitol | 1 (mM) | |
| 4 | 1 | 1 | PMSF | 0.002 (%(w/v)) | |
| 5 | 1 | 1 | sodium azide | 0.01 (%(w/v)) | |
| 6 | 1 | 1 | ADP | 1 (mM) |
