1KME
CRYSTAL STRUCTURE OF BACTERIORHODOPSIN CRYSTALLIZED FROM BICELLES
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2001-04-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.000, 108.900, 55.900 |
Unit cell angles | 90.00, 113.58, 90.00 |
Refinement procedure
Resolution | 50.000 * - 2.000 |
R-factor | 0.263 |
Rwork | 0.263 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c3w |
RMSD bond length | 0.008 |
RMSD bond angle | 1.150 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.102 | 0.260 |
Total number of observations | 99274 * | |
Number of reflections | 31440 | |
<I/σ(I)> | 11.2 | 3.2 |
Completeness [%] | 92.8 | 62.5 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 3.5 | 37 * | sodium phosphate, DMPC, Chapso, pH 3.5, VAPOR DIFFUSION, HANGING DROP, Bicelle Method, temperature 310.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | bR | 8.0 (mg/ml) | |
2 | 1 | drop | bicell | 8 (%) | |
3 | 1 | drop | beta-octylglucoside | 2.5 (%) | |
4 | 1 | reservoir | sodium phosphate | 3.2 (M) | pH3.5 |