1KMB
SELECTIN-LIKE MUTANT OF MANNOSE-BINDING PROTEIN A
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-03-08 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 79.700, 84.900, 98.700 |
Unit cell angles | 90.00, 105.30, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.100 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rtm |
RMSD bond length | 0.008 |
RMSD bond angle | 22.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.54) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.053 | 0.276 |
Number of reflections | 36862 | |
<I/σ(I)> | 13 | 4.5 |
Completeness [%] | 99.5 | 99 |
Redundancy | 2.9 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.8 | 22 * | 8-10% PEG 8000, 2% PEG 1000, 1 MM TRIS-CL, PH 7.8, 200 MM NACL, 20 MM CACL2, 2 MM NAN3. PRIOR TO DATA COLLECTION, THE CRYSTAL WAS ADAPTED TO THE MOTHER LIQUOR PLUS 20% MPD. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2 (mg/ml) | |
10 | 1 | drop | solution A | ||
2 | 1 | drop | 50 (mM) | ||
3 | 1 | drop | 5 (mM) | ||
4 | 1 | reservoir | PEG8000 | 8-10 (%(w/v)) | solution A |
5 | 1 | reservoir | PEG1000 | 2 (%) | solution A |
6 | 1 | reservoir | Tris-HCl | 100 (mM) | solution A |
7 | 1 | reservoir | 200 (mM) | solution A | |
8 | 1 | reservoir | 2 (mM) | solution A | |
9 | 1 | reservoir | 20 (mM) | solution A |