1KM8
The Structure of a Cytotoxic Ribonuclease From the Oocyte of Rana Catesbeiana (Bullfrog)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 115 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11-26 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.122, 45.645, 68.603 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.140 - 1.900 |
| R-factor | 0.177 |
| Rwork | 0.174 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1onc |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.600 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.140 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.042 | 0.138 |
| Number of reflections | 7650 | |
| <I/σ(I)> | 12.2 | 10.89 |
| Completeness [%] | 96.9 | 88.3 |
| Redundancy | 6.24 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | Ammonium phosphate, Tris hydrochloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
