1KM1

Orotidine monophosphate decarboxylase mutant S127A crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-15
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	58.335, 73.753, 59.399
Unit cell angles	90.00, 119.41, 90.00

Refinement procedure

Resolution	30.000 * - 1.600
R-factor	0.177
Rwork	0.177
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	22.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.700
High resolution limit [Å]	1.600	1.600
Rmerge	0.051 *
Total number of observations	530511 *
Number of reflections	55090
Completeness [%]	95.1	80.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5 *	298	Wu, N., (2000) Acta Crystallogr., Sect.D, 56, 912. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	11 (mg/ml)
2	1	reservoir	trisodium citrate	1.1 (M)	pH6.5
3	1	reservoir	glycerol	3 (%)