1KLX
Helicobacter pylori cysteine rich protein B (hcpB)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Wavelength(s) | 0.9791, 0.9794, 0.8856 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 51.360, 51.360, 205.941 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.950 |
R-factor | 0.19232 |
Rwork | 0.187 |
R-free | 0.23840 * |
Structure solution method | MAD |
RMSD bond length | 0.040 |
RMSD bond angle | 2.438 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.001 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.054 * | |
Number of reflections | 12049 | |
Completeness [%] | 99.8 * | 49.8 * |
Redundancy | 9.3 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 * | 20 * | PEG 8000, sodium citrate, pH 3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 * | 20 * | PEG 8000, sodium citrate, pH 3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4.4 (mg/ml) | |
2 | 1 | drop | sodium acetate | 40 (mM) | |
3 | 1 | drop | EDTA | 1 (mM) | pH5.5 |
4 | 1 | reservoir | PEG8000 | 25 (%) | |
5 | 1 | reservoir | sodium citrate | 0.1 (M) | pH3.0 |