1KLF
FIMH ADHESIN-FIMC CHAPERONE COMPLEX WITH D-MANNOSE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-03-18 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 138.077, 138.130, 215.352 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 * - 2.800* |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.27900 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qun |
RMSD bond length | 0.007 |
RMSD bond angle | 26.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 * | 2.900 |
High resolution limit [Å] | 2.800 * | 2.800 * |
Rmerge | 0.069 | 0.478 |
Total number of observations | 370427 * | |
Number of reflections | 99135 | |
<I/σ(I)> | 13 | 2.7 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 * | 298 | ammonium suplhate, pH 8.20, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4.7 (mg/ml) | |
2 | 1 | drop | MES | 20 (mM) | pH6.5 |
3 | 1 | drop | alpha-D-mannose | 7 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 1.0 (M) | |
5 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.2 |