1KKU
Crystal structure of nuclear human nicotinamide mononucleotide adenylyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-07-02 |
Detector | MARRESEARCH |
Wavelength(s) | 0.934 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 147.459, 147.459, 61.710 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.234 * |
Rwork | 0.236 |
R-free | 0.28600 * |
Structure solution method | MIR |
RMSD bond length | 0.018 |
RMSD bond angle | 2.000 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHELXS |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.075 | |
Number of reflections | 14056 * | |
Completeness [%] | 99.8 | 99 |
Redundancy | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | ammonium sulfate, Tris, isopropanol, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris | 0.02 (M) | pH8.0 |
2 | 1 | drop | ATP | 2 (mM) | |
3 | 1 | drop | 2 (mM) | ||
4 | 1 | drop | protein | 20 (mg/ml) | |
5 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
6 | 1 | reservoir | Tris | 0.1 (M) | pH8.1 |
7 | 1 | reservoir | isopropyl alcohol | 4 (%(v/v)) |