1KJZ

Structure of the large gamma subunit of initiation factor eIF2 from Pyrococcus abyssi-G235D mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-31
Detector	MARRESEARCH
Wavelength(s)	0.934
Spacegroup name	P 1 21 1
Unit cell lengths	51.480, 86.741, 58.083
Unit cell angles	90.00, 110.40, 90.00

Refinement procedure

Resolution	34.000 - 1.850
R-factor	0.225 *
Rwork	0.225
R-free	0.25500
Structure solution method	MIR + MAD
RMSD bond length	0.005
RMSD bond angle	1.300 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.000	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.049 *	0.324 *
Number of reflections	40474
<I/σ(I)>	8	2.2
Completeness [%]	99.1	98.4
Redundancy	4.9	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7 *	297	peg8000, tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop		200 (mM)
3	1	drop	MOPS	10 (mM)	pH6.7
4	1	drop	2-mercaptoethanol	10 (mM)
5	1	reservoir	PEG8000	8-18 (%)
6	1	reservoir	Tris-HCl	0.1 (M)	pH8.5