1KJW
SH3-Guanylate Kinase Module from PSD-95
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 60.509, 60.509, 209.956 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.800 |
| R-factor | 0.23 * |
| Rwork | 0.230 |
| R-free | 0.25000 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.080 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.077 * | 0.351 * |
| Number of reflections | 37032 | |
| Completeness [%] | 99.4 | 94.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | 288 | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 3 | 1 | reservoir | PEG400 | 2 (%) | |
| 4 | 1 | reservoir | HEPES | 10 (mM) | pH7.0 |
