1KJW
SH3-Guanylate Kinase Module from PSD-95
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 60.509, 60.509, 209.956 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.800 |
R-factor | 0.23 * |
Rwork | 0.230 |
R-free | 0.25000 |
Structure solution method | MAD |
RMSD bond length | 0.005 * |
RMSD bond angle | 1.080 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.077 * | 0.351 * |
Number of reflections | 37032 | |
Completeness [%] | 99.4 | 94.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7 | 288 | used microseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 2 (M) | |
3 | 1 | reservoir | PEG400 | 2 (%) | |
4 | 1 | reservoir | HEPES | 10 (mM) | pH7.0 |