1KJV
TAP-B-associated rat MHC class I molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-28 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.95007 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.397, 50.522, 200.073 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 * - 1.480 |
| R-factor | 0.16404 |
| Rwork | 0.162 |
| R-free | 0.19100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ed3 molecule 1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.560 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.500 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.049 * | 0.693 * |
| Number of reflections | 82429 | 2721 * |
| <I/σ(I)> | 32.4 | 2 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 3.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 17 * | 26% PEG 4000, 0.2 M Li2SO4, 2% MPD, 0.1 M Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 26 (%(w/v)) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | MPD | 2 (%(v/v)) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
