1KJT

Crystal Structure of the GABA(A) Receptor Associated Protein, GABARAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9333
Spacegroup name	P 21 21 21
Unit cell lengths	29.720, 56.040, 64.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.235 *
Rwork	0.235
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006 *
RMSD bond angle	1.146 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.050 *	0.439 *
Total number of observations	56243 *
Number of reflections	7790 *
Completeness [%]	99.4 *	95.5 *
Redundancy	3.8 *	3.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.2 *	293	PEG 2000MME, Tris HCL, nickel chloride, sodium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris	20 (mM)	pH7.2
2	1	drop		100 (mM)
3	1	drop	protein	25 (mg/ml)
4	1	reservoir	Tris	0.1 (mM)	pH8.5
5	1	reservoir	PEG2000MME	30 (%(w/v))
6	1	reservoir		5 (mM)