1KJQ
Crystal structure of glycinamide ribonucleotide transformylase in complex with Mg-ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-07-10 |
| Detector | SBC-2 |
| Wavelength(s) | 0.7009 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.350, 179.340, 75.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.050 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.21400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1eyz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 15.600 * |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.090 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.066 | 0.314 |
| Number of reflections | 365075 | 29388 * |
| <I/σ(I)> | 22.2 | 1.9 |
| Completeness [%] | 93.0 | 75.5 |
| Redundancy | 3.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.7 | 4 * | used macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ADP | 5 (mM) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | 250 (mM) | ||
| 4 | 1 | drop | protein | 20 (mM) | |
| 5 | 1 | reservoir | PEG5000 ME | 16-24 (%(w/v)) | |
| 6 | 1 | reservoir | MOPS | 100 (mM) | pH6.7 |
| 7 | 1 | reservoir | 100 (mM) |
