1KJM
TAP-A-associated rat MHC class I molecule
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-04-04 |
Detector | MARRESEARCH |
Wavelength(s) | 1.08 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 110.049, 109.566, 45.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.800 - 2.350 |
R-factor | 0.24935 |
Rwork | 0.247 |
R-free | 0.26800 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ED3 MOLECULE 1 |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.520 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.800 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.080 * | 0.423 * |
Number of reflections | 22313 | 2120 * |
<I/σ(I)> | 12.7 | 1.86 |
Completeness [%] | 94.9 | 92 |
Redundancy | 2.8 * | 2.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 17 * | 2M (NH4)2SO4, 1% glycerol, 0.1 M Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 2 (M) | |
3 | 1 | reservoir | glycerol | 1 (%(v/v)) | |
4 | 1 | reservoir | Tris-HCl | 0.1 (M) | p8.5 |