1KJ9
Crystal structure of purt-encoded glycinamide ribonucleotide transformylase complexed with Mg-ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | AREA DETECTOR |
| Collection date | 2000-04-27 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.300, 179.500, 75.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.18 |
| Rwork | 0.182 |
| R-free | 0.23000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1eyz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 16.400 * |
| Data scaling software | SAINT |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.670 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.054 | 0.203 |
| Number of reflections | 106671 | 11879 * |
| <I/σ(I)> | 17.6 | 3.6 |
| Completeness [%] | 95.0 | 79 |
| Redundancy | 2.3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.7 | 4 * | used macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ATP | 5 (mM) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | 250 (mM) | ||
| 4 | 1 | drop | protein | 20 (mM) | |
| 5 | 1 | reservoir | PEG5000 ME | 16-24 (%(w/v)) | |
| 6 | 1 | reservoir | MOPS | 100 (mM) | pH6.7 |
| 7 | 1 | reservoir | 100 (mM) |
