1KIX
Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-09-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 284.000, 284.000, 284.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.700 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Alpha N-terminal domains from alpha-beta-ssDNA complex (1OTC) |
RMSD bond length | 0.007 |
RMSD bond angle | 1.340 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.760 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.075 | 0.548 |
Total number of observations | 135022 * | |
Number of reflections | 27195 | |
<I/σ(I)> | 16.1 | 2 |
Completeness [%] | 98.3 | 94.5 |
Redundancy | 5 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Ammonium Sulfate, Xylitol, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Ammonium Sulfate | ||
2 | 1 | 1 | Xylitol | ||
3 | 1 | 1 | HEPES |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 14 (mg/ml) | |
2 | 1 | drop | oligonucleotide | 20-50 (%) | molar excess |
3 | 1 | reservoir | ammonium sulfate | 2.0-2.1 (M) | |
4 | 1 | reservoir | xylitol | 5 (%(w/v)) | |
5 | 1 | reservoir | HEPES | 50 (mM) | pH7.5 |
6 | 1 | reservoir | dithiothreitol | 2 (mM) | |
7 | 1 | reservoir | sodium azide | 0.02 (%(w/v)) |