1KIV

RECOMBINANT KRINGLE IV-10/M66 VARIANT OF HUMAN APOLIPOPROTEIN(A)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	130
Detector technology	IMAGE PLATE
Collection date	1997-10
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	24.610, 45.680, 63.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.000 - 2.100
R-factor	0.178 *
Rwork	0.178
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	0.032
Data reduction software	RIGAKU (R-AXIS)
Data scaling software	R-AXIS ((RIGAKU))
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.000	2.250
High resolution limit [Å]	2.100	2.100
Rmerge	0.062	0.153 *
Total number of observations	9644 *
Number of reflections	3822
<I/σ(I)>	10.7	3
Completeness [%]	81.0	64
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	acetone	10 (%)
3	1	reservoir	PEG8000	15 (%)
4	1	reservoir	HEPES	0.1 (M)
5	1	reservoir		0.1 (M)