1KIL
Three-dimensional structure of the complexin/SNARE complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-09-20 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.489, 60.425, 159.787 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.230 - 2.500 * |
| R-factor | 0.237 * |
| Rwork | 0.245 |
| R-free | 0.30300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sfc |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 16.600 * |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.200 | 2.340 |
| High resolution limit [Å] | 2.500 * | 2.500 * |
| Rmerge | 0.046 * | 0.169 * |
| Total number of observations | 78881 * | |
| Number of reflections | 14111 * | |
| <I/σ(I)> | 24.8 | 4.3 |
| Completeness [%] | 99.6 * | 99.4 * |
| Redundancy | 5.3 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | 27%(v/v) Iso-Propanol, 200mM MgCl2, 100mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 20 (mM) | pH7.5 |
| 2 | 1 | drop | 130 (mM) | ||
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | reservoir | isopropanol | 35 (%(v/v)) | |
| 5 | 1 | reservoir | 200 (mM) | ||
| 6 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
