1KHP
Monoclinic form of papain/ZLFG-DAM covalent complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.440, 48.930, 52.690 |
| Unit cell angles | 90.00, 112.42, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.185 |
| Rwork | 0.180 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ppn |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.025 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.123 | 0.328 |
| Total number of observations | 57694 * | |
| Number of reflections | 12041 | |
| <I/σ(I)> | 6.6 | 2 |
| Completeness [%] | 88.9 | 75.1 |
| Redundancy | 4.8 | 1.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.2 | 292 | 68% methanol/ethanol (2:1), 34 mM NaCl, 50 mM 2-aminoethanol/HCl, pH 9.2, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 34 (mM) | ||
| 2 | 1 | reservoir | 2-aminoethanol-HCl | 50 (mM) | pH9.2 |
| 3 | 1 | drop | protein | 30 (mg/ml) |
