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1KHN

E. COLI ALKALINE PHOSPHATASE MUTANT (D153HD330N) ZINC FORM

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE BM02
Synchrotron siteESRF
BeamlineBM02
Temperature [K]292
Detector technologyCCD
Collection date1996-07-01
DetectorMARRESEARCH
Wavelength(s)0.98
Spacegroup nameP 63 2 2
Unit cell lengths163.511, 163.511, 138.025
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution10.000 - 2.600
R-factor0.207
Rwork0.207
R-free0.24800
Structure solution methodFOURIER SYNTHESIS
Starting model (for MR)1kh4
RMSD bond length0.012

*

RMSD bond angle1.783

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareX-PLOR (3.1)
Refinement softwareX-PLOR (3.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0002.740
High resolution limit [Å]2.6002.600
Rmerge0.090

*

0.460

*

Number of reflections53663
Completeness [%]96.3

*

92
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

*

8292ammonium sulfate, magnesium chloride, zinc sulfate, TRIS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoirammonium sulfate2.4 (M)
21reservoir10 (mM)
31reservoir1 (mM)
41reservoirTris-HCl100 (mM)pH8.0
51dropprotein30-40 (mg/ml)

222036

PDB entries from 2024-07-03

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