1KHH
Crystal Structure of Guanidinoacetate Methyltransferase from Rat Liver: A Template Structure of Protein Arginine Methyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2001-05-01 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 |
Unit cell lengths | 54.250, 54.250, 156.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.500 |
R-factor | 0.214 * |
Rwork | 0.214 |
R-free | 0.28400 |
Structure solution method | SIR |
RMSD bond length | 0.009 |
RMSD bond angle | 1.280 |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.500 | 2.580 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.047 | 0.139 |
Total number of observations | 109295 * | |
Number of reflections | 15472 * | |
<I/σ(I)> | 11.4 | 5 |
Completeness [%] | 98.0 | 83.4 |
Redundancy | 6.7 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | MES | 50 (mM) | pH6.5 |
2 | 1 | reservoir | dithiothreitol | 1 (mM) | |
3 | 1 | reservoir | SAH | 2 (mM) | |
4 | 1 | reservoir | GAA | 1 (mM) | |
5 | 1 | reservoir | PEG8000 | 8 (%(w/v)) | |
6 | 1 | drop | protein | 10 (mg/ml) |