1KHH

Crystal Structure of Guanidinoacetate Methyltransferase from Rat Liver: A Template Structure of Protein Arginine Methyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2001-05-01
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 43
Unit cell lengths	54.250, 54.250, 156.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.214 *
Rwork	0.214
R-free	0.28400
Structure solution method	SIR
RMSD bond length	0.009
RMSD bond angle	1.280
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.500	2.580
High resolution limit [Å]	2.500	2.500
Rmerge	0.047	0.139
Total number of observations	109295 *
Number of reflections	15472 *
<I/σ(I)>	11.4	5
Completeness [%]	98.0	83.4
Redundancy	6.7	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	4 *	PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MES	50 (mM)	pH6.5
2	1	reservoir	dithiothreitol	1 (mM)
3	1	reservoir	SAH	2 (mM)
4	1	reservoir	GAA	1 (mM)
5	1	reservoir	PEG8000	8 (%(w/v))
6	1	drop	protein	10 (mg/ml)