1KHD
Crystal Structure Analysis of the anthranilate phosphoribosyltransferase from Erwinia carotovora at 1.9 resolution (current name, Pectobacterium carotovorum)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 200 |
Detector technology | AREA DETECTOR |
Collection date | 2001-06-27 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.870, 82.342, 200.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 * - 1.860 |
Rwork | 0.223 |
R-free | 0.26330 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kgz |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.200 * |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 * | |
High resolution limit [Å] | 1.860 | |
Rmerge | 0.063 | 0.372 * |
Total number of observations | 107405 * | |
Number of reflections | 99609 | |
<I/σ(I)> | 38.6 | |
Completeness [%] | 92.0 * | |
Redundancy | 25. * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 3000, MnCl2, glutamine, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 10 (mM) | pH6.5 |
10 | 1 | reservoir | PRPP | 1.25 (mM) | |
11 | 1 | reservoir | anthranilate | 0.0012 (mM) | |
12 | 1 | reservoir | dithiothreitol | 1 (mM) | |
13 | 1 | reservoir | EDTA | 1 (mM) | |
2 | 1 | drop | glycerol | 10 (%) | |
3 | 1 | drop | dithiothreitol | 1 (mM) | |
4 | 1 | drop | EDTA | 1 (mM) | |
5 | 1 | drop | protein | 20 (mg/ml) | |
6 | 1 | reservoir | PEG3000 | 25 (%) | |
7 | 1 | reservoir | HEPES | 0.2 (M) | pH6.5 |
8 | 1 | reservoir | glutamine | 4.5 (mM) | |
9 | 1 | reservoir | 10.0 (mM) |