1KH9

E. COLI ALKALINE PHOSPHATASE MUTANT (D153GD330N) COMPLEX WITH PHOSPHATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	292
Collection date	1996-07
Detector	SDMS
Spacegroup name	P 63 2 2
Unit cell lengths	164.010, 164.010, 138.878
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.196
Rwork	0.196
R-free	0.25000
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1kh7
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.640
High resolution limit [Å]	2.500	2.500
Number of reflections	32077
Completeness [%]	83.4	80.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	292	ammonium sulfate, magnesium chloride, zinc sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K