1KH6

Crystal Structure of an RNA Tertiary Domain Essential to HCV IRES-mediated Translation Initiation.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Wavelength(s)	0.9204, 0.9197, 0.9273, 0.9020
Spacegroup name	P 42 21 2
Unit cell lengths	66.532, 66.532, 95.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.900
R-factor	0.279 *
Rwork	0.283
R-free	0.28300 *
RMSD bond length	0.006 *
RMSD bond angle	1.220 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800	2.800 *
Rmerge	0.078 *	0.536 *
Total number of observations	72167 *
Number of reflections	9652
Completeness [%]	98.3 *	95.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6 *	303	30% MPD, 0.1 M sodium citrate pH 5.6, 0.2 M ammonium acetate, 2.5 mM magnesium chloride, and 1 mM spermidine, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 303K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MPD
2	1	1	sodium citrate
3	1	1	ammonium acetate
4	1	1	MgCl
5	1	1	spermidine

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	MPD	30 (%(v/v))
3	1	reservoir	sodium citrate	0.1 (M)	pH5.6
4	1	reservoir	ammonium acetate	0.2 (M)