1KH2
Crystal Structure of Thermus thermophilus HB8 Argininosuccinate Synthetase in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-09-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 |
| Unit cell lengths | 228.910, 228.910, 161.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.900 * - 2.300 |
| R-factor | 0.2087 * |
| Rwork | 0.209 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.430 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.074 * | 0.270 * |
| Total number of observations | 685640 * | |
| Number of reflections | 143708 | |
| Completeness [%] | 99.9 | 99.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | tris, ammonium sulfate, glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 2.4 (M) | |
| 3 | 1 | reservoir | glycerol | 30 (%(v/v)) | |
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5 |
| 5 | 1 | reservoir | ATP | 10 (mM) | |
| 6 | 1 | reservoir | 10 (mM) |
