1KGZ
Crystal Structure Analysis of the Anthranilate Phosphoribosyltransferase from Erwinia carotovora (current name, Pectobacterium carotovorum)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 200 |
| Detector technology | AREA DETECTOR |
| Collection date | 2000-08-07 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.378, 81.686, 101.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.440* |
| Rwork | 0.214 |
| R-free | 0.26430 * |
| Structure solution method | MIR |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.200 * |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.067 * | 0.398 * |
| Number of reflections | 20457 * | |
| <I/σ(I)> | 37.6 | 7.1 |
| Completeness [%] | 98.7 * | 98.6 |
| Redundancy | 3.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 3000, MnCl2, glutamine, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | pH6.5 |
| 10 | 1 | reservoir | PRPP | 1.25 (mM) | |
| 11 | 1 | reservoir | anthranilate | 0.0012 (mM) | |
| 12 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 13 | 1 | reservoir | EDTA | 1 (mM) | |
| 2 | 1 | drop | glycerol | 10 (%) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | protein | 20 (mg/ml) | |
| 6 | 1 | reservoir | PEG3000 | 25 (%) | |
| 7 | 1 | reservoir | HEPES | 0.2 (M) | pH6.5 |
| 8 | 1 | reservoir | glutamine | 4.5 (mM) | |
| 9 | 1 | reservoir | 10.0 (mM) |
