1KGT

Crystal Structure of Tetrahydrodipicolinate N-Succinyltransferase in Complex with Pimelate and Succinyl-CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1999-04-02
Detector	XENTRONICS
Wavelength(s)	1.5418
Spacegroup name	H 3
Unit cell lengths	95.980, 95.980, 73.440
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	99.000 - 2.300
R-factor	0.171
Rwork	0.168
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.024
RMSD bond angle	2.200
Data reduction software	X-GEN
Data scaling software	XDS
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.500
High resolution limit [Å]	2.300	2.300
Rmerge	0.072	0.339
Total number of observations	47749 *
Number of reflections	11389
Completeness [%]	99.8	99.9
Redundancy	4.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	298	Binder, D.A., (1996) Proteins, 26, 115. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	HEPES	20 (mM)	pH7.5
3	1	reservoir	PEG4000	12 (%)
4	1	reservoir	ammonium sulfate	200 (mM)
5	1	reservoir	MES	100 (mM)	pH6.2
6	1	reservoir	CoA	2.5 (mM)
7	1	reservoir	2-aminopimelate	16 (mM)