1KGP
R2F from Corynebacterium Ammoniagenes in its Mn substituted form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-04-29 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.670, 91.196, 137.270 |
Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.000 |
R-factor | 0.177 * |
Rwork | 0.177 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.210 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.071 | 0.209 |
Total number of observations | 149319 * | |
Number of reflections | 77244 | |
Completeness [%] | 91.1 | 85.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | PEG4000, sodium citrate, ammonium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.5 |
3 | 1 | reservoir | PEG4000 | 30 (%(w/v)) | |
4 | 1 | reservoir | sodium citrate | 100 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 200 (mM) | pH6-6.5 |