1KGJ

Rat transthyretin (also called prealbumin) complex with 3',5'-dibromoflavone (EMD21388)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	82.545, 82.545, 161.741
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.300
R-factor	0.207
Rwork	0.203
R-free	0.28800
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	TETRAMER OF RAT TTR PDB ENTRY 1GKE
RMSD bond length	0.008 *
RMSD bond angle	1.296 *
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.500	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.102	0.367
Number of reflections	23846
<I/σ(I)>	5.9	2.1
Completeness [%]	92.8	81.1
Redundancy	4.86	2.57

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	ammonium sulphate, acetate buffer, pH 5.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	55-65 (%)
2	1	reservoir	acetate	0.1 (M)	pH5.0