1KGB

structure of ground-state bacteriorhodopsin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-05-29
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97
Spacegroup name	P 63
Unit cell lengths	60.987, 60.987, 109.131
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.650
R-factor	0.177 *
Rwork	0.130
R-free	0.25300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c3w
RMSD bond length	0.017
RMSD bond angle	0.034
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.700
High resolution limit [Å]	1.650	1.650
Rmerge	0.036	0.358
Number of reflections	26695
<I/σ(I)>	32.8	3.2
Completeness [%]	98.7	96.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.6		3.0M Na/K Phosphate, pH 5.6, mono-olein cubic phase, temperature 100K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	OG	2 (%)
2	1	1	sodium potassium phosphate	3.0 (M)	pH5.6