1KG9
Structure of a "mock-trapped" early-M intermediate of bacteriorhosopsin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-06-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | P 63 |
Unit cell lengths | 60.886, 60.886, 109.591 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.810 |
R-factor | 0.221 * |
Rwork | 0.165 |
R-free | 0.29300 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c3w |
RMSD bond length | 0.007 * |
RMSD bond angle | 0.020 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.810 |
Rmerge | 0.050 | 0.410 |
Number of reflections | 20519 | |
<I/σ(I)> | 26.1 | 5.2 |
Completeness [%] | 97.5 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 5.6 | 293 | 3.0M Na/K Phosphate, pH 5.6, mono-olein cubic phase, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | OG | 2 (%) | |
2 | 1 | 1 | sodium potassium phosphate | 3.0 (M) | pH5.6 |