1KG8

X-ray structure of an early-M intermediate of bacteriorhodopsin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-04-22
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 63
Unit cell lengths	60.745, 60.745, 108.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.222 *
Rwork	0.168
R-free	0.29000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c8s
RMSD bond length	0.008
RMSD bond angle	0.022
Data scaling software	SCALEPACK
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.050	0.450 *
Number of reflections	14618
Completeness [%]	95.0	90 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.6	293	3.0M Na/K Phosphate, pH 5.6, mono-olein cubic phase, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	OG	2 (%)
2	1	1	sodium potassium phosphate	3.0 (M)	pH5.6