1KG5
Crystal structure of the K142Q mutant of E.coli MutY (core fragment)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-09-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.490, 50.120, 70.380 |
| Unit cell angles | 90.00, 122.68, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.350 |
| Rwork | 0.128 |
| R-free | 0.16900 |
| Structure solution method | Difference fourier |
| Starting model (for MR) | 1kg2 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.031 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 49330 | |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 91.7 | 55.4 |
| Redundancy | 4.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 288 | Lithium sulfate, HEPES, Sodium chloride, Magnesium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
