1KG4
Crystal structure of the K142A mutant of E. coli MutY (core fragment)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.030, 50.060, 69.880 |
| Unit cell angles | 90.00, 122.60, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| Rwork | 0.149 |
| R-free | 0.19700 |
| Structure solution method | difference fourier |
| Starting model (for MR) | 1kg2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.024 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 30985 | |
| <I/σ(I)> | 17.7 | |
| Completeness [%] | 96.7 | 70.9 |
| Redundancy | 4.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 288 | Lithium sulfate, HEPES, Sodium chloride, Magnesium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
