1KFW
Structure of catalytic domain of psychrophilic chitinase B from Arthrobacter TAD20
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-05-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8007 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 114.280, 114.280, 102.780 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.210 - 1.740 |
| R-factor | 0.1741 |
| Rwork | 0.174 |
| R-free | 0.18900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.850 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 75975 | |
| Completeness [%] | 95.2 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | Ammonium sulfate, MES, additive NAD, pH 6.0, VAPOR DIFFUSION, HANGING DROP at 295K |
