1KFQ

Crystal Structure of Exocytosis-Sensitive Phosphoprotein, pp63/parafusin (Phosphoglucomutse) from Paramecium. OPEN FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-09-06
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	64.900, 90.600, 212.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.227 *
Rwork	0.233
R-free	0.28600 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.300 *
Data scaling software	XDS
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.138 *	0.443 *
Total number of observations	182091 *
Number of reflections	46866	3280 *
Completeness [%]	94.6	58.4
Redundancy	3.9	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	290	PEG-MME 2000, ammonium tartrate, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	20 (mM)	pH7.5
2	1	drop	protein	10 (mg/ml)
3	1	reservoir	sodium tartrate instead of ammonium sulfate	0.2-0.4 (M)