1KFK
Crystal structure of Tryptophan Synthase From Salmonella Typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.316, 59.357, 67.406 |
| Unit cell angles | 90.00, 94.53, 90.00 |
Refinement procedure
| Resolution | 19.510 - 2.400 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.22500 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.300 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 28891 | |
| Completeness [%] | 89.2 | 50.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 296 | PEG 8000, EDTA, spermine, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
