1KFI
Crystal Structure of the Exocytosis-Sensitive Phosphoprotein, pp63/Parafusin (phosphoglucomutase) from Paramecium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-08-18 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8345 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.346, 133.609, 150.545 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
R-factor | 0.233 * |
Rwork | 0.227 |
R-free | 0.28400 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 * |
Data scaling software | XDS |
Phasing software | BRUTE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.041 * | 0.085 * |
Total number of observations | 180300 * | |
Number of reflections | 50610 | 4304 * |
Completeness [%] | 94.2 | 77.6 |
Redundancy | 3.6 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 290 | 18 %PEG-MME 2000, 0.2M ammonium sulfate, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
2 | 1 | drop | protein | 10 (mg/ml) | |
3 | 1 | reservoir | PEG2000MME | 18 (%(v/v)) | |
4 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
5 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |