1KFI

Crystal Structure of the Exocytosis-Sensitive Phosphoprotein, pp63/Parafusin (phosphoglucomutase) from Paramecium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-08-18
Detector	MARRESEARCH
Wavelength(s)	0.8345
Spacegroup name	P 21 21 21
Unit cell lengths	67.346, 133.609, 150.545
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.233 *
Rwork	0.227
R-free	0.28400 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.300 *
Data scaling software	XDS
Phasing software	BRUTE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.041 *	0.085 *
Total number of observations	180300 *
Number of reflections	50610	4304 *
Completeness [%]	94.2	77.6
Redundancy	3.6	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	290	18 %PEG-MME 2000, 0.2M ammonium sulfate, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	20 (mM)	pH7.5
2	1	drop	protein	10 (mg/ml)
3	1	reservoir	PEG2000MME	18 (%(v/v))
4	1	reservoir	ammonium sulfate	0.2 (M)
5	1	reservoir	sodium acetate	0.1 (M)	pH4.6