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1KFI

Crystal Structure of the Exocytosis-Sensitive Phosphoprotein, pp63/Parafusin (phosphoglucomutase) from Paramecium

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsEMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron siteEMBL/DESY, HAMBURG
BeamlineBW7B
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date1998-08-18
DetectorMARRESEARCH
Wavelength(s)0.8345
Spacegroup nameP 21 21 21
Unit cell lengths67.346, 133.609, 150.545
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution30.000 - 2.400
R-factor0.233

*

Rwork0.227
R-free0.28400

*

Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.007
RMSD bond angle1.300

*

Data scaling softwareXDS
Phasing softwareBRUTE
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0002.490
High resolution limit [Å]2.4002.400
Rmerge0.041

*

0.085

*

Total number of observations180300

*

Number of reflections506104304

*

Completeness [%]94.277.6
Redundancy3.62.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.5

*

29018 %PEG-MME 2000, 0.2M ammonium sulfate, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropTris-HCl20 (mM)pH7.5
21dropprotein10 (mg/ml)
31reservoirPEG2000MME18 (%(v/v))
41reservoirammonium sulfate0.2 (M)
51reservoirsodium acetate0.1 (M)pH4.6

217705

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