1KF1
Structure and Packing of Human Telomeric DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2001-03-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.901 |
| Spacegroup name | P 6 |
| Unit cell lengths | 56.682, 56.682, 42.106 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.100 |
| R-factor | 0.226 * |
| Rwork | 0.229 |
| R-free | 0.26200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k8p |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.015 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.052 | 0.117 |
| Total number of observations | 50951 * | |
| Number of reflections | 4595 * | |
| <I/σ(I)> | 50 | 15 |
| Completeness [%] | 98.0 * | 97.8 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 285 | PEG 400, potassium iodide, potassium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | KI | ||
| 3 | 1 | 1 | KCl |
