1KF0
Crystal Structure of Pig Muscle Phosphoglycerate Kinase Ternary Complex with AMP-PCP and 3PG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 297 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.600, 110.300, 48.000 |
| Unit cell angles | 90.00, 93.90, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.2 |
| Rwork | 0.173 |
| R-free | 0.25240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pig muscle PGK ternary complex with MnAMP-PNP and 3-PG May et al. Proteins 24:292-303 1996) |
| RMSD bond length | 0.004 |
| RMSD bond angle | 27.050 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.081 | 0.252 |
| Number of reflections | 12274 | |
| Completeness [%] | 90.9 * | 63.18 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 15 * | magnesium chloride, PEG 8000, PH 7.0, 288K, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | AMP-PCP | 10 (mM) | |
| 2 | 1 | reservoir | 12 (mM) | ||
| 3 | 1 | reservoir | 3PG | 10 (mM) | |
| 4 | 1 | reservoir | PEG8000 | 27-28 (%(w/w)) | pH7.0 |
