1KF0
Crystal Structure of Pig Muscle Phosphoglycerate Kinase Ternary Complex with AMP-PCP and 3PG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 297 |
Detector technology | IMAGE PLATE |
Collection date | 1996-04-03 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.600, 110.300, 48.000 |
Unit cell angles | 90.00, 93.90, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.2 |
Rwork | 0.173 |
R-free | 0.25240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pig muscle PGK ternary complex with MnAMP-PNP and 3-PG May et al. Proteins 24:292-303 1996) |
RMSD bond length | 0.004 |
RMSD bond angle | 27.050 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.081 | 0.252 |
Number of reflections | 12274 | |
Completeness [%] | 90.9 * | 63.18 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 15 * | magnesium chloride, PEG 8000, PH 7.0, 288K, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | AMP-PCP | 10 (mM) | |
2 | 1 | reservoir | 12 (mM) | ||
3 | 1 | reservoir | 3PG | 10 (mM) | |
4 | 1 | reservoir | PEG8000 | 27-28 (%(w/w)) | pH7.0 |