1KEY

Crystal Structure of Mouse Testis/Brain RNA-binding Protein (TB-RBP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-06-15
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	97.246, 135.457, 91.228
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.650
Rwork	0.247
R-free	0.29000
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.080 *
Data scaling software	SCALEPACK
Phasing software	DM
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.650
Rmerge	0.045 *	0.457 *
Total number of observations	193026 *
Number of reflections	34219
Completeness [%]	95.3	98.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	Pascal, J.M., (2001) Acta Cryst., D57, 1692. *
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	Pascal, J.M., (2001) Acta Cryst., D57, 1692. *