1KDJ

OXIDIZED FORM OF PLASTOCYANIN FROM DRYOPTERIS CRASSIRHIZOMA

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	298
Detector technology	DIFFRACTOMETER
Collection date	1996-02-20
Detector	WEISSENBERG
Spacegroup name	P 61
Unit cell lengths	73.118, 73.118, 31.095
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 1.700
R-factor	0.241 *
Rwork	0.234
R-free	0.28800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	POPLAR PLASTOCYANIN
RMSD bond length	0.013
RMSD bond angle	27.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.089 *	0.148
Total number of observations	69003 *
Number of reflections	10220 *
<I/σ(I)>	9.1	2.45
Completeness [%]	96.0 *	83.3
Redundancy	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5	20 *	pH 4.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	sodium acetate	0.1 (M)
3	1	drop	ammonium sulfate	32 (%sat)
4	1	reservoir	sodium azide	1 (mM)
5	1	reservoir	ammonium sulfate	64-65 (%sat)