1KDD

X-ray structure of the coiled coil GCN4 ACID BASE HETERODIMER ACID-d12La16I BASE-d12La16L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2000-07-19
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0093
Spacegroup name	P 41 21 2
Unit cell lengths	86.278, 86.278, 78.864
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.140
R-factor	0.252 *
Rwork	0.252
R-free	0.30220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	calculated structure
RMSD bond length	0.004
RMSD bond angle	13.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.060	0.257
Number of reflections	18925
Completeness [%]	97.5	96.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	293	PEG 4000, Na Hepes, 2-propanol, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG4000	18-20 (%)
3	1	reservoir	sodium HEPES	0.1 (M)	pH7.7
4	1	reservoir	2-propanol	10 (%)