1KD8
X-RAY STRUCTURE OF THE COILED COIL GCN4 ACID BASE HETERODIMER ACID-d12Ia16V BASE-d12La16L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-10-19 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9763 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 86.044, 86.044, 78.384 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 1.900 |
R-factor | 0.232 * |
Rwork | 0.232 |
R-free | 0.27600 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | calculated structure |
RMSD bond length | 0.018 * |
RMSD bond angle | 0.990 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.060 | 0.413 |
Number of reflections | 25471 | |
Completeness [%] | 98.9 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 293 | PEG 4000, Na Hepes, 2-propanol, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 18-20 (%) | |
3 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.7 |
4 | 1 | reservoir | 2-propanol | 10 (%) |