1KD7
Crystal structure of an extracellular domain fragment of human BAFF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-12-12 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 |
Unit cell lengths | 121.720, 121.720, 160.740 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.222 |
Rwork | 0.217 |
R-free | 0.25400 * |
Structure solution method | MIR |
RMSD bond length | 0.008 * |
RMSD bond angle | 1.440 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.079 | 0.267 |
Number of reflections | 31743 | |
<I/σ(I)> | 14.2 | 2.9 |
Completeness [%] | 95.6 | 81.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | 8% PEG 4000, 0.1 M sodium acetatesolution of 8% PEG 4000, 0.1 M sodium acetate pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | 8% PEG 4000, 0.1 M sodium acetatesolution of 8% PEG 4000, 0.1 M sodium acetate pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9 (mg/ml) | |
2 | 1 | drop | sodium phosphate | 150 (mM) | pH7. |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | reservoir | PEG4000 | 8 (%(w/v)) | |
5 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.5 |